Proline, abbreviated as Pro or P, is a cyclic sub amino acid. Once entering the peptide chain, hydroxylation can occur, resulting in the formation of 4-hydroxyproline, which is an important component of animal collagen. Hydroxyproline is also present in various plant proteins, particularly related to the formation of cell walls. Plants often experience significant accumulation of proline under various stresses such as drought, high temperature, low temperature, and salinity. It is widely used in clinical, biomaterials, industry, and other fields.
There are three forms of proline: DL proline, L-proline, and D-proline. Proline, commonly referred to as L-proline, is a naturally occurring amino acid that forms columnar crystals at room temperature.
1.Brief introduction of L-Proline:
Proline, abbreviated as Pro or P, is a cyclic sub amino acid. Once entering the peptide chain, hydroxylation can occur, resulting in the formation of 4-hydroxyproline, which is an important component of animal collagen. Hydroxyproline is also present in various plant proteins, particularly related to the formation of cell walls. Plants often experience significant accumulation of proline under various stresses such as drought, high temperature, low temperature, and salinity. It is widely used in clinical, biomaterials, industry, and other fields.
There are three forms of proline: DL proline, L-proline, and D-proline. Proline, commonly referred to as L-proline, is a naturally occurring amino acid that forms columnar crystals at room temperature.
2.Physical and chemical properties of L-Proline:
Appearance: White crystalline or crystalline powder; Slightly odorous, slightly sweet in taste, easily deliquescent but not easily crystallized.
Other names: 2-Pyrrolidin-2-carboxylic acid, Pyrrolidin-2-carboxylic acid
Abbreviation: P, Pro
Chemical formula: C5H9NO2
Molecular weight: 115.13
Molar mass: 115.13g/mol
CAS: 147-85-3
EINECS: 205-702-2
PSA: 49.33000
LogP: 0.15180
Density: 1.35
Melting point: 228 ° C (dec.) (lit.)
Boiling point: 252.2 º C at 760 mmHg
Flash point: 106.3 º C
Refractive index: -85 ° (C=4, H2O)
Water solubility: easily soluble in water (about 162g in 100g water at 25 ℃), soluble in ethanol, insoluble in ether or n-butanol.
Storage conditions: Room temperature
Isoelectric point (pI): 6.30
3.Application of L-Proline 99%:
(1).Plant cold resistance:
Proline (Pro) is one of the components of plant proteins and can be widely present in the plant body in a free state. Under stress conditions such as drought and salinity, many plants accumulate a large amount of proline in their bodies. The accumulated proline not only serves as an osmotic regulator in plant cytoplasm, but also plays an important role in stabilizing the structure of biological macromolecules, reducing cell acidity, relieving ammonia toxicity, and regulating cell redox as an energy reservoir.
Under adverse conditions (drought, saline alkali, heat, cold, freezing), the content of proline in plants significantly increases. The content of proline in plants reflects their stress resistance to a certain extent, and varieties with strong drought resistance often accumulate more proline. Therefore, measuring proline content can serve as a physiological indicator for drought resistance breeding. In addition, due to its strong hydrophilicity, proline can stabilize protoplast colloids and metabolic processes within tissues, thereby reducing the freezing point and preventing cell dehydration. Under low temperature conditions, the increase of proline in plant tissues can enhance the cold resistance of plants, and therefore can also be used as a physiological indicator for cold resistance breeding.
(2).Intrabiological effects:
In living organisms, proline is not only an ideal osmoregulatory substance, but also serves as a protective substance for membranes and enzymes, as well as a free radical scavenger, thereby affecting plants
4.Storage and shelf-life: 24momths at dry,shade and room temperatures;
